DNA

Part:BBa_K4449012:Design

Designed by: SRIJANI SAHA , RASHMI RANJAN BEHERA , SATYAM KUMAR SINGH   Group: iGEM22_IISER_Berhampur   (2022-10-07)


Aptamer sequences


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Here in the 40 random nucleotide sequences, a ratio of 3:2:2:3 is followed between A:T:G:C.

Source

The Aptamer sequences were generated using python with the help of Jupyter Notebook. We have added a sequence from the library in the registry page as an example. To know more details about the rest 499 sequences please visit "https://docs.google.com/spreadsheets/d/1MICJ94mhiy_pHvqC_b31TbRaymPHyPen/edit?usp=sharing&ouid=114372498163057334768&rtpof=true&sd=true"

References


1.Tao Wang , Wang Yin,Hadi AlShamaileh , Yumei Zhang , Phuong Ha-Lien Tran , Tuong Ngoc-Gia Nguyen , Yong Li , Kuisheng Chen , Miaomiao Sun, Yingchun Hou , Weihong Zhang, Qingxia Zhao, Changying Chen , Pei-Zhuo Zhang, and Wei Duan, 2019 Feb;30(1),A detailed protein-SELEX protocol allowing visual assessments of individual steps for high success rate, DOI: 10.1089/hgtb.2018.237